3 articles published in JoVE
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II Grace H. Hwang*1, Jessica L. Hopkins*1, Philip W. Jordan1 1Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model Stephen R Wellard*1, Jessica Hopkins*1, Philip W. Jordan1 1Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health The goal of this tubule squash technique is to rapidly assess cytological features of developing mouse spermatocytes while preserving cellular integrity. This method allows for the study of all stages of spermatogenesis, and can be easily implemented alongside other biochemical and molecular biological approaches for the study of mouse meiosis.
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body Quigly Dragotakes1, Arturo Casadevall1 1Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health This technique describes an automated batch image processor designed to measure polysaccharide capsule and body radii. While initially designed for Cryptococcus neoformans capsule measurements the automated image processor can also be applied to other contrast based detection of circular objects.