Oak Ridge Institute for Science and Education 4 articles published in JoVE Environment The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide Melissa Anne Johnson1,2, Robert Hollingsworth2, Samuel Fortna2,3, Luis F. Aristizábal4, Nicholas C. Manoukis2 1Oak Ridge Institute for Science and Education, 2Daniel K. Inouye US Pacific Basin Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, 3College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 4Independent Consultant on CBB Management Comprehensive monitoring of coffee berry borer and host plant dynamics is essential for aggregating landscape-level data to improve management of this invasive pest. Here, we present a protocol for scientific monitoring of coffee berry borer movement, infestation, mortality, coffee plant phenology, weather, and farm management via a mobile electronic data recording application. Engineering Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory Vincent H. Hammond1, Billy C. Hornbuckle1, Anit K. Giri1, Anthony J. Roberts1, Thomas L. Luckenbaugh2, Joseph M. Marsico3, Scott M. Grendahl1, Kristopher A. Darling1 1Weapons and Materials Research Directorate, US Army Research Laboratory, 2Bowhead Total Enterprise Solutions, LLC, 3Oak Ridge Institute for Science and Education This paper provides a brief overview of the ongoing efforts at the Army Research Laboratory on the processing of bulk nanocrystalline metals with an emphasis on the methodologies used for the production of the novel metal powders. Immunology and Infection Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens Swinburne A. J. Augustine1, Tarsha N. Eason2, Kaneatra J. Simmons1, Clarissa L. Curioso3, Shannon M. Griffin1, Malini K. D. Ramudit1, Trevor R. Plunkett4 1National Exposure Research Laboratory, U.S. Environmental Protection Agency, 2National Risk Management Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education, 4Department of Biological Sciences, McMicken College of Arts and Sciences, University of Cincinnati In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens simultaneously. Chemistry Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis Derek D. Lovingood1, Jeffrey R. Owens2, Michael Seeber3, Konstantin G. Kornev3, Igor Luzinov3 1Oak Ridge Institute for Science and Education, 2Air Force Research Laboratory, Airbase Technology Division, 3School of Materials Science and Engineering, Clemson University Silica nanoparticles were prepared using acid-catalysis of a siloxane precursor and microwave-assisted synthetic techniques resulting in the controlled growth of nanomaterials ranging from 30-250 nm in diameter. The growth dynamics can be controlled by varying the initial silicic acid concentration, time of the reaction, and temperature of reaction.
