2 articles published in JoVE
High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury John G. Lehman1, Robert D. Causey1, Cristina V. LaGrasta1, Albert L. Ruff1 1US Army Medical Research Institute of Chemical Defense High throughput small inhibitory RNA screening is an important tool that could help to more rapidly elucidate the molecular mechanisms of chemical cornea epithelial injury. Herein, we present the development and validation of exposure models and methods for the high throughput screening of hydrogen fluoride- and chloropicrin-induced cornea epithelial injury.
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons Kyle Hubbard1, Phillip Beske1, Megan Lyman1, Patrick McNutt1 1Research Division, Cellular Molecular Biology Branch, United States Army Medical Research Institute of Chemical Defense A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.