3 articles published in JoVE
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays Barry W. Neun1, Marina A. Dobrovolskaia1 1Nanotechnology Characterization Lab., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles.
Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications Matthew A. Ellis1, Giovanna Grandinetti2, Katye M. Fichter1 1Department of Chemistry, Missouri State University, 2Center for Molecular Microscopy, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research In this protocol, the synthesis of Cd-free InP/ZnS quantum dots (QDs) is detailed. InP-based QDs are gaining popularity due to the toxicity of Cd2+ ions that may be released through nanoparticle degradation. After synthesis, QDs are solubilized in water using an amphiphilic polymer for use in biomedical applications.
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors Krishna P. Kota1,6, Veronica Soloveva2,6,7, Laura M. Wanner3,6, Glenn Gomba4,6, Erkan Kiris3,5,6, Rekha G. Panchal6, Christopher D. Kane2,6,7, Sina Bavari6 1Perkin Elmer Inc., 2Henry M. Jackson Foundation, 3The Geneva Foundation, 4ORISE, 5Frederick National Laboratory for Cancer Research, 6Division of Molecular and Translational Sciences, US Army Medical Research Institute of Infectious Diseases, 7DoD Biotechnology High Performance Computing Software Applications Institute (BHSAI), Telemedicine and Advanced Technology Research Center (TATRC), US Army Medical Research and Materiel Command (USAMRMC) Botulinum neurotoxin is one of the most potent toxins among Category-A biothreat agents, yet a post-exposure therapeutic is not available. The high content imaging approach is a powerful methodology for identifying novel inhibitors as it enables multiparameter screening using biologically relevant motor neurons, the primary target of this toxin.