4 articles published in JoVE
Incorporation of a Survivable Liver Biopsy Procedure in Mice to Assess Non-alcoholic Steatohepatitis (NASH) Resolution Stephanie Oldham1, Christian Rivera2, Michelle L. Boland1, James L. Trevaskis1 1Cardiovascular, Renal and Metabolic Diseases, MedImmune, 2Laboratory Animal Resources, MedImmune In clinical settings, a liver biopsy is used to assess stages of non-alcoholic steatohepatitis (steatosis, inflammation, hepatocyte ballooning, and fibrosis). Here, we illustrate a survivable liver biopsy in mice which can be used for histological analyses to enable assessment of therapeutic agents in a manner more aligned with clinical trials.
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry Maria Letizia Giardino Torchia1, Raffaello Cimbro2 1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens Michael Surace1, Karma DaCosta1, Anna Huntley1, Weiguang Zhao1, Christopher Bagnall2, Charles Brown1, Chichung Wang3, Kristin Roman3, Jennifer Cann1, Arthur Lewis2, Keith Steele1, Marlon Rebelatto1, Edwin R. Parra4, Clifford C. Hoyt3, Jaime Rodriguez-Canales1 1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays Jacqueline Naylor1, Alessandra Rossi1, Christopher Brankin2, David C. Hornigold1 1Cardiovascular & Metabolic Disease, MedImmune, 2Lab Automation and Support, MedImmune Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.