Loyola University Maryland 1 article published in JoVE Biology Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines Derek M. Kendig1,2, Norm R. Hurst1, John R. Grider1 1Department of Physiology and Biophysics, Virginia Commonwealth University, 2Department of Biology, Loyola University Maryland Recently available video recording and spatiotemporal mapping (STmap) techniques make it possible to visualize and quantify both propagating and mixing patterns of intestinal motility. The goal of this protocol is to explain the generation and analysis of STmaps using the GastroIntestinal Motility Monitoring (GIMM) system.