Uniformed Services University 3 articles published in JoVE Cancer Research Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment Dave Mitchell*1,2, Allison L. Hunt*1,4, Kelly A. Conrads1,2, Brian L. Hood1,2, Sasha C. Makohon-Moore1,2, Christine Rojas1, G. Larry Maxwell1,3,4, Nicholas W. Bateman1,2,3, Thomas P. Conrads1,3,4 1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes. Developmental Biology Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging Kelsey M. Sheard1, Rachel T. Cox1 1Department of Biochemistry and Molecular Biology, Uniformed Services University The objective of this protocol is to use live imaging to visualize the effects of oxidative damage on the localization and dynamics of subcellular structures in Drosophila ovaries. Developmental Biology Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells Orion Furmanski1,2, Michael D. Nieves1,2,3, Martin L. Doughty1,2,3 1Center for Neuroscience and Regenerative Medicine, Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics, Uniformed Services University, 3Graduate Program in Neuroscience, Uniformed Services University This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.