1 article published in JoVE
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity Jesse Zhan1, Ruchi Komal1, William T. Keenan2, Samer Hattar1, Diego C. Fernandez1 1Section on Light and Circadian Rhythms (SLCR), National Institute of Mental Health, 2Howard Hughes Medical Institute, Department of Neuroscience, Scripps Research Institute Here we describe two non-invasive methods to chronically control neuronal activity using chemogenetics in mice. Eye-drops were used to deliver clozapine-N-oxide (CNO) daily. We also describe two methods for prolonged administration of CNO in drinking water. These strategies for chronic neuronal control require minimal intervention reducing animals’ stress.