Leidos Biomedical Research, Inc. 1 article published in JoVE Cancer Research Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model Robert Y. S. Cheng1, Nimit L. Patel2, Timothy Back1, Debashree Basudhar1, Veena Somasundaram1, Joseph D. Kalen2, David A. Wink1, Lisa A. Ridnour1 1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc. This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.