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3 articles published in JoVE
Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse Ruby Boateng1, Nathaniel Boechat1, Philipp P. Henrich1, Ewelina Bolcun-Filas1 1The Jackson Laboratory Here, we present an optimized protocol for imaging entire ovaries for quantitative and qualitative analyses using whole-mount immunostaining, multiphoton microscopy, and 3D visualization and analysis. This protocol accommodates high-throughput, reliable, and repeatable processing that is applicable for toxicology, clinical diagnostics, and genomic assays of ovarian function.
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons Yun Li1, Brittany D. Roy1, Wei Wang1, Lifeng Zhang1, Stephen B. Sampson1, Da-Ting Lin1 1The Jackson Laboratory By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window Lifeng Zhang*1, Andree Lapierre*1, Brittany Roy1, Maili Lim1, Jennifer Zhu1, Wei Wang1, Stephen B. Sampson1, Kyuson Yun1, Bonnie Lyons1, Yun Li1, Da-Ting Lin1 1The Jackson Laboratory By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.