Colby College 3 articles published in JoVE Developmental Biology Injecting Gryllus bimaculatus Eggs Samantha K. Barry1, Taro Nakamura2, Yuji Matsuoka3, Christoph Straub4, Hadley W. Horch4, Cassandra G. Extavour5 1Colby College, 2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 3Department of Biological Sciences, National University of Singapore, 4Department Biology and Department of Neuroscience, Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic manipulation. Genetics Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput Grace Uwase1, Taylor P. Enrico1, David S. Chelimo1, Benjamin R. Keyser1, Russell R. Johnson1 1Department of Biology, Colby College An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure. Neuroscience Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson's Disease Model of Drosophila Jennifer Liao1, Laura W. Morin1, S. Tariq Ahmad1 1Department of Biology, Colby College Parkinson’s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, causing locomotion defects. Rotenone models Parkinson’s disease in Drosophila. This paper outlines two assays that characterize both spontaneous and startle-induced locomotion deficiencies caused by rotenone.
