MDI Biological Laboratory 1 article published in JoVE Developmental Biology Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy Thomas S. Lisse1, Elizabeth A. Brochu1, Sandra Rieger1 1Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
