3 articles published in JoVE
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts Tina T. Thomas1, Sahiti Chukkapalli1, Raelene A. Van Noord1, Melanie Krook2, Mark J. Hoenerhoff3, Jonathan R. Dillman4, Elizabeth R. Lawlor2,5, Valerie P. Opipari5, Erika A. Newman1 1Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine, The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School Here, we present a protocol to utilize ultrasound-guided injection of neuroblastoma (NB) and Ewing's sarcoma (ES) cells (established cell lines and patient-derived tumor cells) at biologically relevant sites to create reliable preclinical models for cancer research.
Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres Flor M. Mendez1, Felipe J. Núñez1,2, Rocío I. Zorrilla-Veloz3,4, Pedro R. Lowenstein1,2, Maria G. Castro1,2 1Department of Cell and Developmental Biology, University of Michigan Medical School, 2Department of Neurosurgery, University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, 4Department of Biology, University of Puerto Rico-Río Piedras Campus Epigenetic mechanisms are frequently altered in glioma. Chromatin immunoprecipitation could be used to study the consequences of genetic alterations in glioma that result from changes in histone modifications which regulate chromatin structure and gene transcription. This protocol describes native chromatin immunoprecipitation on murine brain tumor neurospheres.
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers Hussein S. Hamid1, John M. Hayes2, Eva L. Feldman2, Stephen I. Lentz3 1University of Michigan Medical School, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, University of Michigan This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.