Biointerfaces Institute, University of Michigan 1 article published in JoVE Developmental Biology Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs) Richard H. Barnes II1, Tae-Hwa Chun1 1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, Biointerfaces Institute, University of Michigan We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.
