2 articles published in JoVE
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation Linyuan Ma*1, Lydia Jang*1, Jian Chen2, Jun Song1, Dongshan Yang1, Jifeng Zhang1, Y. Eugene Chen1, Jie Xu1 1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Celetrix LLC Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer Yin Wang1, Mark L. Day2, Diane M. Simeone3, Phillip L. Palmbos1 1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.