Henry Ford Health System 3 articles published in JoVE Biology Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint Thomas Wilson1, Navdeep Kaur1, Jason Davis2, Shabana Amanda Ali1,3 1Bone and Joint Center, Henry Ford Health System, 2Department of Orthopedic Surgery, Henry Ford Health System, 3Center for Molecular Medicine and Genetics, Wayne State University Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis. Medicine Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock Shuktika Nandkeolyar1, Poonam Velagapudi2, Mir B. Basir3, Aditya S. Bharadwaj1 1Division of Cardiology, Loma Linda University Medical Center, 2Division of Cardiology, University of Nebraska, 3Division of Cardiology, Henry Ford Health System Percutaneous ventricular assist devices are increasingly being utilized in patients with acute myocardial infarction and cardiogenic shock. Herein, we discuss the mechanism of action and hemodynamic effects of such devices. We also review algorithms and best practices for the implantation, management and weaning of these complex devices. Bioengineering Measuring 3D In-vivo Shoulder Kinematics using Biplanar Videoradiography Rebekah L. Lawrence1, Roger Zauel1, Michael J. Bey1 1Bone and Joint Center, Department of Orthopaedic Surgery, Henry Ford Health System Biplane videoradiography can quantify shoulder kinematics with a high degree of accuracy. The protocol described herein was specifically designed to track the scapula, humerus, and the ribs during planar humeral elevation, and outlines the procedures for data collection, processing, and analysis. Unique considerations for data collection are also described.
