2 articles published in JoVE
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta Naimisha Beeravolu1,2, Christina McKee1,2, Ali Alamri1,2, Sasha Mikhael3, Christina Brown1,2, Mick Perez-Cruet2,4, G. Rasul Chaudhry1,2 1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
RNAi Interference by dsRNA Injection into Drosophila Embryos Ekaterini Iordanou1, Rachana R. Chandran1, Nicholas Blackstone1, Lan Jiang1 1Department of Biological Sciences, Oakland University RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.