University of Minnesota - Twin Cities 4 articles published in JoVE Biochemistry Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking Santosh Adhikari1, Chiranjib Banerjee*1, Joe Moscatelli*1,2, Elias M. Puchner1 1School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College Conventional BODIPY conjugates can be used for live-cell single-molecule localization microscopy (SMLM) through exploitation of their transiently forming, red-shifted ground state dimers. We present an optimized SMLM protocol to track and resolve subcellular neutral lipids and fatty acids in living mammalian and yeast cells at the nanoscopic length scale. Behavior Experimental Paradigm for Measuring the Effects of Self-distancing in Young Children Amanda Grenell1, Rachel E. White2, Emily O. Prager1, Catherine Schaefer1, Ethan Kross3, Angela L. Duckworth4, Stephanie M. Carlson1 1Insitute of Child Development, University of Minnesota-Twin Cities, 2Department of Psychology, Hamilton College, 3Department of Psychology, University of Michigan, 4Department of Psychology, University of Pennsylvania An experimental paradigm was created to measure the effects of self-distancing in young children (4-6-year-olds). Self-distancing is a process through which individuals adopt a less egocentric perspective. This paradigm has been used to examine the effects of self-distancing on young children's self-regulation. Cancer Research Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol Tanner J. McArdle1, Brenda M. Ogle1,2,3,4,5, Felicite K. Noubissi6 1Department of Biomedical Engineering, University of Minnesota - Twin Cities, 2Stem Cell Institute, University of Minnesota - Twin Cities, 3Masonic Cancer Center, University of Minnesota - Twin Cities, 4Lillehei Heart Institute, University of Minnesota - Twin Cities, 5Institute for Engineering in Medicine, University of Minnesota - Twin Cities, 6Department of Biology, Jackson State University This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells. Chemistry Ethylene Polymerizations Using Parallel Pressure Reactors and a Kinetic Analysis of Chain Transfer Polymerization Ryan J. Hue1, Ian A. Tonks1 1Department of Chemistry, University of Minnesota - Twin Cities A protocol for the high-throughput analysis of polymerization catalyst, chain transfer polymerizations, polyethylene characterization, and reaction kinetic analysis is presented.