2 articles published in JoVE
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation Charles J. Benck1, Tijana Martinov2, Brian T. Fife2, Devavani Chatterjea1 1Department of Biology, Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology, University of Minnesota This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.
Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus Tijana Martinov1, Madison Mack1, Akilah Sykes1, Devavani Chatterjea1 1Biology Department, Macalester College Electronic von Frey measurements are an effective and improved alternative to classical von Frey filaments, as they allow rapid and precise measurement of changes in rodent mechanical withdrawal thresholds to continuously applied pressure stimuli before and after an inflammatory event.