University of North Carolina at Charlotte View Institution's Website 6 articles published in JoVE Immunology and Infection Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry Kristen E. Funk1, Sarah K. Lotz1 1Department of Biological Sciences, University of North Carolina at Charlotte This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis. Genetics Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers Mengxiao Ma1, Shreya Goyal2, Christopher G. Burd1, Richard J. Chi2 1Department of Cell Biology, Yale School of Medicine, 2Department of Biological Sciences, University of North Carolina at Charlotte Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast. Chemistry Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III) Jacob M. Dixon1, Shunji Egusa1 1Department of Physics and Optical Science, Center for Biomedical Engineering & Science, University of North Carolina The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented. Engineering An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids Jerry Dahlberg1, Peter T. Tkacik1, Brigid Mullany1, Eric Fleischhauer1, Hossein Shahinian1, Farzad Azimi1, Jayesh Navare1, Spencer Owen1, Tucker Bisel1, Tony Martin1, Jodie Sholar1, Russell G. Keanini1 1Department of Mechanical Engineering, University of North Carolina at Charlotte An experimentally accessible analog method for studying molecular hydrodynamic processes in dense fluids is presented. The technique uses particle image velocimetry of vibrated, high-restitution grain piles and allows direct, macroscopic observation of dynamical processes known and predicted to exist in strongly interacting, high density gases and liquids. Chemistry Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles Andrew Tobias1, Song Qing2, Marcus Jones1 1Department of Chemistry, University of North Carolina at Charlotte, 2Nanosys Inc. The synthesis of uniform gold nanoparticles coated with semiconductor shells of CdS or ZnS is performed. The semiconductor coating is conducted by first depositing a silver sulfide shell and exchanging the silver cations for zinc or cadmium cations. Biology Study of the DNA Damage Checkpoint using Xenopus Egg Extracts Jeremy Willis*1, Darla DeStephanis*1, Yogin Patel1, Vrushab Gowda1, Shan Yan1 1Department of Biology, University of North Carolina at Charlotte Xenopus egg extract is a useful model system to investigate the DNA damage checkpoint. This protocol is for the preparation of Xenopus egg extracts and DNA damage checkpoint inducing reagents. These techniques are adaptable to a variety of DNA damaging approaches in the study of the DNA damage checkpoint signaling.
