2 articles published in JoVE
A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation Jayanth S. Shankara Narayanan1, Partha Ray1, Ibtehaj Naqvi2, Rebekah White1 1Moores Cancer Center, University California San Diego, 2Duke University School of Medicine Irreversible electroporation (IRE) is a non-thermal ablation technique used for the treatment of locally advanced pancreatic cancer. Being a relatively new technique, the effects of IRE on the tumor growth are poorly understood. We have developed a syngeneic mouse model that facilitates studying the effects of IRE on pancreatic cancer.
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells Sriram Vijayraghavan1, Boris Kantor1 1Duke Viral Vector Core, Department of Neurobiology, Duke University School of Medicine We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.