2 articles published in JoVE
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue Thomas Lampl1, Jo A. Crum1, Taylor A. Davis1, Carol Milligan2,3,4, Victoria Del Gaizo Moore1 1Chemistry Department, Elon University, 2Department of Neurobiology and Anatomy, Wake Forest School of Medicine, 3Neuroscience Graduate Program, Wake Forest School of Medicine, 4ALS Center Translational Science Unit, Wake Forest School of Medicine Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.
A Neuroscientific Approach to the Examination of Concussions in Student-Athletes Caroline J. Ketcham1, Eric Hall1, Walter R. Bixby1, Srikant Vallabhajosula2, Stephen E. Folger2, Matthew C. Kostek3, Paul C. Miller1, Kenneth P. Barnes1, Kirtida Patel4 1Department of Exercise Science, Elon University, 2Department of Physical Therapy Education, Elon University, 3Department of Physical Therapy, Duquesne University, 4Department of Sports Medicine, Elon University There is great variability in an individual’s risk for concussion and their corresponding recovery. A multifaceted approach to concussion evaluation is warranted; including baseline testing of athletes before participation in sport and timely evaluation post injury. The goal of this protocol is to provide an appropriate multifaceted approach to examine concussions.