FirstString Research, Inc. 1 article published in JoVE Medicine Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR Melissa A. Abt1, Christina L. Grek2, Gautam S. Ghatnekar2, Elizabeth S. Yeh1 1Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 2FirstString Research, Inc. This protocol describes how to use quantitative Real-time PCR (QRT-PCR) to detect tumor cell specific mRNA representing metastasis within the mouse lung tissue.