UNC Neuroscience Center 2 articles published in JoVE Neuroscience Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips Smita R. Paranjape1,2, Tharkika Nagendran1,2, Valerie Poole3, Joseph Harris3, Anne Marion Taylor1,2,3 1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, 3Xona Microfluidics, LLC This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining. Neuroscience Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons Tharkika Nagendran1,2, Valerie Poole3, Joseph Harris3, Anne Marion Taylor1,2,3 1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, UNC, 3Xona Microfluidics, LLC This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.
