3 articles published in JoVE
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes Meihua Wan1,2, Pouya Amrollahi2,3, Dali Sun4, Christopher Lyon2,3, Tony Y. Hu2,3 1Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, 3School of Biological and Health Systems Engineering, Arizona State University, 4Department of Electrical and Computer Engineering, North Dakota State University Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.
A Precise and Autonomous System for the Detection of Insect Emergence Patterns Meghan M. Bennett1,2, Joseph P. Rinehart3, George D. Yocum3, Ian Yocum3 1Department of Biological Sciences, North Dakota State University, 2School of Life Sciences, Arizona State University, 3Red River Valley Agricultural Research Center, USDA-ARS Measurement of insect emergence patterns requires precision. Existing systems are only semi-automated and sample size is limited. We addressed these issues by designing a system using microcontrollers to precisely measure the time of emergence of large numbers of emerging insects.
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm Aurelia C. Kucera1, Britt J. Heidinger2 1Environmental and Conservation Sciences Program, North Dakota State University, 2Department of Biological Sciences, North Dakota State University This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.