University of Nebraska - Kearney 1 article published in JoVE Biochemistry Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes April Maschmann1, Cody Masters1, Melissa Davison1, Joshua Lallman1, Drew Thompson1, Kristy L. Kounovsky-Shafer1 1Department of Chemistry, University of Nebraska - Kearney Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.