Plymouth State University 1 article published in JoVE Immunology and Infection Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates Kyle D. Brumfield*1, Bailey M. Carignan*1, Jordan N. Ray1, Panagiota E. Jumpre1, Mike S. Son1 1Department of Biological Sciences, Plymouth State University This manuscript describes proper Vibrio cholerae maintenance techniques in addition to a series of biochemical assays, collectively utilized for quick and reliable differentiation between clinical and environmental V. cholerae biotypes in a laboratory setting.