3 articles published in JoVE
Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture Shawna M. Rotoli1, Salvatore J. Caradonna1 1Department of Molecular Biology, Rowan University, School of Osteopathic Medicine and Graduate School of Biomedical Sciences Disulfide linkages have long been known to stabilize the structure of many proteins. A simple method to analyze multimeric complexes stabilized by these linkages is through non-reducing SDS-PAGE analysis. Here, this method is illustrated by analyzing the nuclear isoform of dUTPase from the human bone osteosarcoma cell line U-2 OS.
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response Stephen D. Willis1, A. K. M. Nawshad Hossian2, Nathan Evans2, Mark J. Hickman2 1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment Hao Wu1, Ana R. Rodriguez2, Bernd W. Spur2, Venkat Venkataraman1,2 1Graduate School of Biomedical Sciences, Rowan University, 2Department of Cell Biology, Rowan University School of Osteopathic Medicine A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4.