2 articles published in JoVE
High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately Wenjin Chen2,3, Chung Wong1,3, Evan Vosburgh1,3, Arnold J. Levine3,4, David J. Foran2,3, Eugenia Y. Xu1,3 1Raymond and Beverly Sackler Foundation, New Jersey, 2Histopathology and Imaging Shared Resource, Rutgers University, 3Rutgers Cancer Institute of New Jersey, Rutgers University, 4School of Natural Sciences, Institute for Advanced Study, New Jersey We present a high-throughput image analysis software application to measure the size of three-dimensional tumor spheroids imaged with bright-field microscopy. This application provides a fast and effective way to examine the effects of therapeutic drugs on spheroids, which is beneficial for researchers who wish to use spheroids in drug screens.
Conducting Miller-Urey Experiments Eric T. Parker1, James H. Cleaves2,3, Aaron S. Burton4, Daniel P. Glavin5, Jason P. Dworkin5, Manshui Zhou1, Jeffrey L. Bada6, Facundo M. Fernández1 1School of Chemistry and Biochemistry, Georgia Institute of Technology, 2Earth-Life Science Institute, Tokyo Institute of Technology, 3Institute for Advanced Study, 4Astromaterials Research and Exploration Science Directorate, NASA Johnson Space Center, 5Goddard Center for Astrobiology, NASA Goddard Space Flight Center, 6Geosciences Research Division, Scripps Institution of Oceanography, University of California at San Diego The Miller-Urey experiment was a pioneering study regarding the abiotic synthesis of organic compounds with possible relevance to the origins of life. Simple gases were introduced into a glass apparatus and subjected to an electric discharge, simulating the effects of lightning in the primordial Earth’s atmosphere-ocean system. The experiment was conducted for one week, after which, the samples collected from it were analyzed for the chemical building blocks of life.