Stevens Institute of Technology 4 articles published in JoVE Bioengineering Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue Seyed Mohammad Mir1, Jiawen Chen1, Meghan R. Pinezich1, John D. O’Neill3, Brandon A. Guenthart4, Gordana Vunjak-Novakovic2, Jinho Kim1 1Department of Biomedical Engineering, Stevens Institute of Technology, 2Department of Biomedical Engineering, Columbia University, 3Department of Cell Biology, State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery, Stanford University The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct. Biology 3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation Christina Li1, Jeel Shah1, Kylee Wrath1, Dahlia Matouba1, Connor Mills1, Kishore Punnath2, Ansu Perekatt1 1Department of Biology and Chemical Biology, Stevens Institute of Technology, 2Department of Pharmacology, Pennsylvania State University College of Medicine The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential. Immunology and Infection Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes Wei Hu1,2, Xiang Song1, Haibo Yu1, Jingyu Sun2, Yong Zhao1 1Center for Discovery and Innovation, Hackensack Meridian Health Center, 2Department of Chemistry and Chemistry Biology, Stevens Institute of Technology Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages. Behavior Characterization of the Sense of Agency over the Actions of Neural-machine Interface-operated Prostheses Jonathon S. Schofield1, Courtney E. Shell1, Zachary C. Thumser1,2, Dylan T. Beckler1, Raviraj Nataraj3, Paul D. Marasco1,4 1Laboratory for Bionic Integration, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 2Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 3Department of Biomedical Engineering, Stevens Institute of Technology, 4Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center Here we present a protocol which characterizes the sense of agency developed over the control of sensate virtual or robotic prosthetic hands. Psychophysical questionnaires are employed to capture the explicit experience of agency, and time interval estimates (intentional binding) are employed to implicitly measure the sense of agency.
