3 articles published in JoVE
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons David A. Clark1,2, Donovan Kohler1, America Mathis1, Eryn Slankster1, Samipya Kafle1, Seth R. Odell1,2, Dennis Mathew1,2 1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.
Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice Andrew B. Nuss1, Manoj G. Mathew2, Monika Gulia-Nuss2 1Department of Agriculture, Nutrition, and Veterinary Sciences, University of Nevada, Reno, 2Department of Biochemistry and Molecular Biology, University of Nevada, Reno The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.
A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis Michele M. Pelter1, Teri M. Kozik2, Denise L. Loranger1, Mary G. Carey3 1Orvis School of Nursing, University of Nevada, Reno, 2The State University of New York at Buffalo, St. Joseph's Medical Center, 3Strong Memorial Hospital, University of Rochester Medical Center Continuous 12-lead electrocardiographic (ECG) monitoring can identify transient myocardial ischemia, even when asymptomatic, among patients with suspected acute coronary syndrome (ACS). In this article we describe our method for initiating patient monitoring using a Holter device, downloading the ECG data for off-line analysis, and how to utilize the ECG software to identify transient ischemia.