3 articles published in JoVE
Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ Bernard T. Drumm1, Grant W. Hennig2, Salah A. Baker1, Kenton M. Sanders1 1Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, 2Department of Pharmacology, The Robert Larner, M.D. College of Medicine, University of Vermont Genetically encoded Ca2+ indicators (GECIs) have radically changed how in situ Ca2+ imaging is performed. To maximize data recovery from such recordings, appropriate analysis of Ca2+ signals is required. The protocols in this paper facilitate the quantification of Ca2+ signals recorded in situ using spatiotemporal mapping and particle-based analysis.
A Mouse Model of Intestinal Partial Obstruction Se Eun Ha*1, Lai Wei*1, Brian G. Jorgensen*1, Moon Young Lee*2, Paul J. Park1, Sandra M. Poudrier1, Seungil Ro1 1Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University Intestinal obstructions are a partial or complete blockage of the intestine that can cause severe abdominal pain, nausea, vomiting, and preventing the passage of stool. This procedure for creating intestinal partial obsructions in mice is reliable in studying the mechanisms underlying pathological cell growth and death in the intestine.
Isolation and Cannulation of Cerebral Parenchymal Arterioles Paulo W. Pires1, Fabrice Dabertrand2, Scott Earley1 1Department of Pharmacology, University of Nevada School of Medicine, 2Department of Pharmacology, University of Vermont College of Medicine This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.