8 articles published in JoVE

• Behavior

  
  Lexical Decision Task for Studying Written Word Recognition in Adults with and without Dementia or Mild Cognitive Impairment Alexandre Nikolaev¹, Eve Higby^2,3, JungMoon Hyun⁴, Sameer Ashaie^{5,6 1}Helsinki Collegium for Advanced Studies, University of Helsinki, ²University of California, Riverside, ³California State University, East Bay, ⁴Hunter College, City University of New York, ⁵Feinberg School of Medicine, Northwestern University, ⁶Shirley Ryan AbilityLab This article describes how to implement a simple lexical decision experiment to assess written word recognition in neurologically healthy participants and in individuals with dementia and cognitive decline. We also provide a detailed description of reaction time analysis using principal components analysis (PCA) and mixed-effects modeling.

• Chemistry

  
  Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates Maria Davydova¹, Guillaume Dewaele Le Roi^1,2, Pierre Adumeau¹, Brian M. Zeglis^{1,2,3,4 1}Department of Chemistry, Hunter College of the City University of New York, ²Ph.D. Program in Chemistry, Graduate Center of the City University of New York, ³Department of Radiology, Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology, Weill Cornell Medical College In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

• Chemistry

  
  Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction Rosemery Membreno^1,2, Brendon E. Cook^1,2,3, Brian M. Zeglis^{1,2,3,4 1}Department of Chemistry, Hunter College of the City University of New York, ²Ph.D. Program in Chemistry, Graduate Center of the City University of New York, ³Department of Radiology, Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology, Weill Cornell Medical College This protocol describes the synthesis and characterization of a trans-cyclooctene (TCO)-modified antibody and a ¹⁷⁷Lu-labeled tetrazine (Tz) radioligand for pretargeted radioimmunotherapy (PRIT). In addition, it details the use of these two constructs for in vivo biodistribution and longitudinal therapy studies in a murine model of colorectal cancer.

• Medicine

  
  A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis Dibash K. Das^1,2, Victoria Durojaiye¹, Adeodat Ilboudo¹, Michelle K. Naidoo¹, Olorunseun Ogunwobi^{1,2 1}Department of Biological Sciences, Hunter College of the City University of New York, ²Departments of Biology and Biochemistry, The Graduate Center of the City University of New York The metastatic spread of cancer is the major cause of cancer-related deaths. We provide an in-depth description of our survival surgery methodology for establishing a “patient-like” orthotopic syngeneic mouse model system for studying the mechanisms of metastasis in solid organ tumors.

• Medicine

  
  Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model Dibash K. Das^1,2, Michelle K. Naidoo¹, Adeodat Ilboudo¹, Pascal DuBois¹, Victoria Durojaiye¹, Chen Liu³, Olorunseun O. Ogunwobi^{1,2 1}Department of Biological Sciences, Hunter College of The City University of New York, ²Departments of Biology and Biochemistry, The Graduate Center of The City University of New York, ³Department of Pathology, Immunology, and Laboratory Medicine, University of Florida Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.

• Biology

  
  Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice Daniella Kovacsics¹, Jayne Raper^{1 1}Department of Biological Sciences, Hunter College, CUNY In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.

• Biology

  
  Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting Ravichandra Bachu*¹, Frances-Camille S. Padlan*², Sara Rouhanifard², Michael Brenowitz², Jörg C. Schlatterer^{2 1}Department of Chemistry, Hunter College, ²Department of Biochemistry, Albert Einstein College of Medicine This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.

• Biology

  
  Blood Collection from the American Horseshoe Crab, Limulus Polyphemus Peter Armstrong^1,2, Mara Conrad^{3 1}Department of Molecular and Cell Biology, University of California, Davis, ²Marine Biological Laboratory - MBL- woods hole, ³Department of Biological Sciences, Hunter College of CUNY The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.