2 articles published in JoVE

• Biology

  
  Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting Ravichandra Bachu*¹, Frances-Camille S. Padlan*², Sara Rouhanifard², Michael Brenowitz², Jörg C. Schlatterer^{2 1}Department of Chemistry, Hunter College, ²Department of Biochemistry, Albert Einstein College of Medicine This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.

• Biology

  
  Blood Collection from the American Horseshoe Crab, Limulus Polyphemus Peter Armstrong^1,2, Mara Conrad^{3 1}Department of Molecular and Cell Biology, University of California, Davis, ²Marine Biological Laboratory - MBL- woods hole, ³Department of Biological Sciences, Hunter College of CUNY The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.