2 articles published in JoVE
Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting Ravichandra Bachu*1, Frances-Camille S. Padlan*2, Sara Rouhanifard2, Michael Brenowitz2, Jörg C. Schlatterer2 1Department of Chemistry, Hunter College, 2Department of Biochemistry, Albert Einstein College of Medicine This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.
Blood Collection from the American Horseshoe Crab, Limulus Polyphemus Peter Armstrong1,2, Mara Conrad3 1Department of Molecular and Cell Biology, University of California, Davis, 2Marine Biological Laboratory - MBL- woods hole, 3Department of Biological Sciences, Hunter College of CUNY The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.