3 articles published in JoVE
High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings Timothy M. Benseman1,2,3, Yang Hao1,2, Vitalii K. Vlasko-Vlasov1, Ulrich Welp1, Alexei E. Koshelev1, Wai-Kwong Kwok1, Ralu Divan4, Courtney Keiser5, Chiharu Watanabe6, Kazuo Kadowaki6 1Materials Science Division, Argonne National Laboratory, 2Department of Physics, University of Illinois at Chicago, 3Department of Physics, CUNY Queens College, 4Center for Nanoscale Materials, Argonne National Laboratory, 5Department of Physics, University of Northern Iowa, 6Institute for Materials Science, University of Tsukuba Europium thenoyltrifluoroacetonate (EuTFC) has an optical luminescence line at 612 nm, whose activation efficiency decreases strongly with temperature. If a sample coated with a thin film of this material is micro-imaged, the 612 nm luminescent response intensity may be converted into a direct map of sample surface temperature.
Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans Jun Liang1, Sheng Xiong2,3, Cathy Savage-Dunn2,3 1Department of Science, Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology, Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center, Queens College, The City University of New York (CUNY) We demonstrate how to use the RNAi feeding technique to knock down target genes and score body size phenotype in C. elegans. This method could be used for a large scale screen to identify potential genetic components of interest, such as those involved in body size regulation by DBL-1/TGF-β signaling.
Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio Corinna Singleman1, Nathalia G. Holtzman1 1Department of Biology, Queens College, City University of New York A clear, standardized method for dissection and isolation of the zebrafish heart at multiple developmental stages are described. Annotation and quantification techniques are also discussed.