3 articles published in JoVE
Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans Shaarika Sarasija1, Kenneth R. Norman1 1Department of Regenerative and Cancer Cell Biology, Albany Medical College Mitochondrial respiration is critical for organismal survival; therefore, oxygen consumption rate is an excellent indicator of mitochondrial health. In this protocol, we describe the use of a commercially available respirometer to measure basal and maximal oxygen consumption rates in live, intact, and freely-motile Caenorhabditis elegans.
Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution Wasay M. Shaikh Qureshi1, Lianjie Miao1, David Shieh1, Jingjing Li1, Yangyang Lu1, Saiyang Hu1, Margarida Barroso1, Joseph Mazurkiewicz2, Mingfu Wu1 1Department of Molecular and Cellular Physiology, Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics, Albany Medical College We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.
Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping N. Jeremy Hill1, Disha Gupta1,2, Peter Brunner1,2, Aysegul Gunduz1,2, Matthew A. Adamo3, Anthony Ritaccio2, Gerwin Schalk1,2,4,5,6,7 1Wadsworth Center, New York State Department of Health, 2Department of Neurology, Albany Medical College, 3Department of Neurosurgery, Albany Medical College, 4Department of Neurosurgery, Washington University, 5Department of Biomed. Eng., Rensselaer Polytechnic Institute, 6Department of Biomed. Sci., State University of New York at Albany, 7Department of Elec. and Comp. Eng., University of Texas at El Paso We present a method for collecting electrocorticographic signals for research purposes from humans who are undergoing invasive epilepsy monitoring. We show how to use the BCI2000 software platform for data collection, signal processing and stimulus presentation. Specifically, we demonstrate SIGFRIED, a BCI2000-based tool for real-time functional brain mapping.