Brookhaven National Laboratory 6 articles published in JoVE Biology Fluorescence Lifetime Macro Imager for Biomedical Applications Rajannya Sen1, Alexander V. Zhdanov1, Ciaran Devoy2, Mark Tangney2, Liisa M. Hirvonen3, Andrei Nomerotski4, Dmitri B. Papkovsky1 1School of Biochemistry and Cell Biology, University College Cork, 2Cancer Research@UCC, University College Cork, 3Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, 4Physics Department, Brookhaven National Laboratory This paper describes the use of a new, fast optical imager for the macroscopic photoluminescence lifetime imaging of long decay emitting samples. The integration, image acquisition, and analysis procedures are described, along with the preparation and characterization of the sensor materials for the imaging and the application of the imager in studying biological samples. Environment A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging Angelia L. Seyfferth1, Matt A. Limmer1, Ryan Tappero2 1Department of Plant and Soil Sciences, University of Delaware, 2National Synchrotron Radiation Lightsource-II, Brookhaven National Laboratory We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging. Engineering Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope Fernando E. Camino1, Vitor R. Manfrinato1, Aaron Stein1, Lihua Zhang1, Ming Lu1, Eric A. Stach1, Charles T. Black1 1Center for Functional Nanomaterials, Brookhaven National Laboratory We use an aberration-corrected scanning transmission electron microscope to define single-digit nanometer patterns in two widely-used electron-beam resists: poly (methyl methacrylate) and hydrogen silsesquioxane. Resist patterns can be replicated in target materials of choice with single-digit nanometer fidelity using liftoff, plasma etching, and resist infiltration by organometallics. Biology The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects Matthew S. Lehnert1, Kristen E. Reiter1, Andrew Bennett1, Patrick D. Gerard2, Qi-Huo Wei3, Miranda Byler1, Huan Yan3, Wah-Keat Lee4 1Department of Biological Sciences, Kent State University at Stark, 2Department of Mathematical Sciences, Clemson University, 3Liquid Crystal Institute, Kent State University, 4Brookhaven National Laboratory Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles. Chemistry Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene Rebecca A. Ryan1, Sophie Williams1, Andrew V. Martin1, Ruben A. Dilanian1, Connie Darmanin2, Corey T. Putkunz1, David Wood3, Victor A. Streltsov4, Michael W.M. Jones5, Naylyn Gaffney6, Felix Hofmann7, Garth J. Williams8, Sebastien Boutet9, Marc Messerschmidt10, M. Marvin Seibert11, Evan K. Curwood11, Eugeniu Balaur2, Andrew G. Peele5, Keith A. Nugent2, Harry M. Quiney1, Brian Abbey2 1ARC Centre of Excellence in Advanced Molecular Imaging, School of Physics, University of Melbourne, 2Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, 3Department of Physics, Imperial College London, 4Florey Institute of Neuroscience and Mental Health, 5Science and Engineering Faculty, Queensland University of Technology, 6Swinburne University of Technology, 7Department of Engineering Science, University of Oxford, 8Brookhaven National Laboratory, 9Linac Coherent Light Source, SLAC National Accelerator Laboratory, 10BioXFEL Science and Technology Center, 11Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, 12Australian Synchrotron We describe an experiment designed to probe the electronic damage induced in nanocrystals of Buckminsterfullerene (C60) by intense, femtosecond pulses of X-rays. The experiment found that, surprisingly, rather than being stochastic, the X-ray induced electron dynamics in C60 are highly correlated, extending over hundreds of unit cells within the crystals1. Engineering Seedless Growth of Bismuth Nanowire Array via Vacuum Thermal Evaporation Mingzhao Liu1, Chang-Yong Nam1, Lihua Zhang1 1Center for Functional Nanomaterials, Brookhaven National Laboratory A protocol for seedless and high yield growth of bismuth nanowire arrays through vacuum thermal evaporation technique is presented.
