3 articles published in JoVE
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models Seth A. Bennett1,2, Samantha N. Cobos1,3, Marcella Meykler1, Michel Fallah1, Navin Rana1, Karen Chen1, Mariana P. Torrente1,4 1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York This protocol outlines experimental procedures to characterize genome-wide changes in the levels of histone post-translational modifications (PTM) occurring in connection with the overexpression of proteins associated with ALS and Parkinson's disease in Saccharomyces cerevisiae models. After SDS-PAGE separation, individual histone PTM levels are detected with modification-specific antibodies via Western blotting.
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid Sylwia A. Dragulska1, Marek T. Wlodarczyk1,2, Mina Poursharifi1,3, John A. Martignetti4,5,6, Aneta J. Mieszawska1,2,3 1Department of Chemistry, Brooklyn College, The City University of New York, 2Ph.D. Program in Chemistry, The Graduate Center of The City University of New York, 3Ph.D. Program in Biochemistry, The Graduate Center of The City University of New York, 4Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 5Women's Health Research Institute, Icahn School of Medicine at Mount Sinai, 6Laboratory for Translational Research, Western Connecticut Health Network This protocol describes an efficient method to synthesize a nanoemulsion of an oleic acids-platinum(II) conjugate stabilized with a lysine-tyrosine-phenylalanine (KYF) tripeptide. The nanoemulsion forms under mild synthetic conditions via self-assembly of the KYF and the conjugate.
Manipulation of Ploidy in Caenorhabditis elegans Erlyana K. Clarke1, Katherine A. Rivera Gomez1,2, Zaki Mustachi1, Mikaela C. Murph1,3, Mara Schvarzstein1,2,3 1Brooklyn College, Biology Department, City University of New York, 2The Graduate Center, Biology Department, City University of New York, 3Advanced Science Research Center, City University of New York This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.