University at Buffalo- SUNY 6 articles published in JoVE Medicine Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research Mark C. Butler1,2, Jack M. Sullivan1,2,3,4,5,6,7 1Research Service, VA Western New York Healthcare System, 2Department of Ophthalmology, (Ross Eye Institute), Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 3Pharmacology/Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 4Physiology/Biophysics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 5Neuroscience Program, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 6The RNA Institute, University at Buffalo- SUNY, 7The SUNY Eye Institute Here we demonstrate a novel approach to using high resolution spectral-domain optical coherence tomography (HR-SD-OCT) to assist delivery of gene therapy agents into the subretinal space, assess its areal coverage, and characterize photoreceptor vitality. Medicine Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury Ravi Alluri1,2, Hilliard L. Kutscher3, Barbara A. Mullan1,2, Bruce A. Davidson1,2, Paul R. Knight1,2 1Department of Anesthesiology, University at Buffalo, State University of New York, 2Department of Anesthsiology, Veterans Admistration Western New York Healthcare System, 3Institute of Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York This protocol induces acute lung injury in a mouse that has close fidelity to the pathogenesis of acid pneumonitis observed in humans. We generate a maximal acute nonlethal low pH lung injury and account for differences in rodent-human anatomic respiratory structure using an open tracheostomy coupled with circumferential pressure release. Bioengineering Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring Maxwell T. Koobatian1, Carmon Koenigsknecht2, Sindhu Row3, Stelios Andreadis3, Daniel Swartz2 1Department of Physiology & Bio-Physics, State University of New York Buffalo School of Medicine, 2Department of Pediatrics, State University of New York Buffalo School of Medicine, 3Department of Chemical and Biological Engineering, State University of New York Buffalo School of Engineering A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice. Medicine Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration Abhishek Kumar1, Arjun Mohan1, Samjot S. Dhillon2, Kassem Harris2 1Division of Pulmonary and Critical Care Medicine, State University of New York, Buffalo, 2Interventional Pulmonology Section, Division of Medicine, Roswell Park Cancer Institute, State University of New York, Buffalo Substernal thyroid lesions are common, and need to be differentiated from malignancy. Obtaining percutaneous fine needle biopsy is not possible due to its retrosternal location. This article proposes a protocol for biopsy of substernal thyroid lesions using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration (EBUS-TBNA). Biology One-channel Cell-attached Patch-clamp Recording Bruce A. Maki1, Kirstie A. Cummings2, Meaghan A. Paganelli1, Swetha E. Murthy3, Gabriela K. Popescu4 1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes. Bioengineering Photoacoustic Cystography Mansik Jeon1,2, Jeehyun Kim3, Chulhong Kim1,2 1Department of Biomedical Engineering, University at Buffalo, The State University of New York, 2Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), 3School of Electrical Engineering and Computer Science, Kyungpook National University Photoacoustic cystography (PAC) has a great potential to map urinary bladders, a radiation sensitive internal organ in pediatric patients, without using any ionizing radiation or toxic contrast agent. Here we demonstrate the use of PAC for mapping urinary bladders with an injection of optical-opaque tracers in rats in vivo.
