2 articles published in JoVE
High Throughput Co-culture Assays for the Investigation of Microbial Interactions Mia I. Temkin1, Caitlin M. Carlson1, Aaron L. Stubbendieck2, Cameron R. Currie1, Reed M. Stubbendieck1 1Department of Bacteriology, University of Wisconsin-Madison, 2Independent Scholar The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format Jack K. Phillips1, Sydney A. Sherman1,2, Sevan R. Oungoulian3, John D. Finan1 1Department of Neurosurgery, NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.