NYU Langone Health 4 articles published in JoVE Biology Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle Qingyue Zhang1,2, Marc A. Kerzhnerman1,2, Nelson García-Vázquez1,2,3, Gergely Rona1,2,4,5 1Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, 2The Laura and Isaac Perlmutter Cancer Center, NYU Langone Health, 3Department of Cell Biology, NYU Grossman School of Medicine, 4Howard Hughes Medical Institute, NYU Grossman School of Medicine, 5Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN Research Centre for Natural Sciences The following protocol presents the detection of single-stranded DNA foci in the G1 phase of the cell cycle utilizing cell cycle synchronization followed by RPA2 immunofluorescent staining. Bioengineering Bioinspired Soft Robot with Incorporated Microelectrodes Ting Wang1,2, Bianca Migliori1,3, Beatrice Miccoli1,4, Su Ryon Shin1 1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2School of Medicine, Jiangsu University, 3Tech4Health and Neuroscience Institutes, NYU Langone Health, 4Department of Electronics and Telecommunication, Politecnico di Torino A bioinspired scaffold is fabricated by a soft photolithography technique using mechanically robust and electrically conductive hydrogels. The micropatterned hydrogels provide directional cardiomyocyte cell alignment, resulting in a tailored direction of actuation. Flexible microelectrodes are also integrated into the scaffold to bring electrical controllability for a self-actuating cardiac tissue. Behavior Efficiently Recording the Eye-Hand Coordination to Incoordination Spectrum John-Ross Rizzo*1,2, Mahya Beheshti*1, James Fung1, Janet C. Rucker2,3, Todd E. Hudson1,2 1Dept. of Rehabilitation Med, New York University Langone Health, 2Dept. of Neurology, New York University Langone Health, 3Dept. of Ophthalmology, New York University Langone Health Cerebral injury can damage both ocular and somatic motor systems. Characterization of motor control post-injury affords biomarkers that assist in disease detection, monitoring, and prognosis. We review a method to measure eye-hand movement control in health and in pathologic incoordination, with look-and-reach paradigms to assess coordination between eye and hand. Cancer Research 3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer Yin Wang1, Mark L. Day2, Diane M. Simeone3, Phillip L. Palmbos1 1Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, 2Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, 3Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.
