2 articles published in JoVE
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors Saranga Naganathan1, Amy Grunbeck1, He Tian1, Thomas Huber1, Thomas P. Sakmar1 1Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University We genetically-encode the unnatural amino acid, p-azido-L-phenylalanine at various targeted positions in GPCRs and show the versatility of the azido group in different applications. These include a targeted photocrosslinking technology to identify residues in the ligand-binding pocket of a GPCR, and site-specific bioorthogonal modification of GPCRs with a peptide-epitope tag or fluorescent probe.
Gavaging Adult Zebrafish Chereen Collymore1,2, Skye Rasmussen2, Ravi J. Tolwani2 1Tri-Institutional Training Program in Laboratory Animal Medicine and Science Memorial Sloan-Kettering Cancer Center, Weill Cornell Medical College, The Rockefeller University, 2Comparative Bioscience Center, The Rockefeller University The increasing use of zebrafish as an animal model requires the development of effective methods for the delivery of known quantities of compounds and solutions. The gavage procedure described below allows for the oral delivery of precise volumes of solution reliably, safely and efficiently to adult zebrafish.