Montefiore Medical Center 3 articles published in JoVE Medicine Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma Kathryn Saturnino1,2,3, Osaro Obanor1,2,3, Cynthia Arvizo4, Julian A. Gingold2,3,5 1Department of Obstetrics & Gynecology and Women's Health, Montefiore Medical Center, 2Albert Einstein College of Medicine, 3Department of OB/GYN, 1400 Pelham Pkwy S, Jacobi Medical Center, 4Division of Minimally Invasive Gynecologic Surgery, Department of OB/GYN, Jacobi Medical Center, 5Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology and Women's Health, Montefiore Medical Center This protocol presents techniques to laparoscopically excise ovarian endometrioma, to perform adhesiolysis with sparing electrosurgical application, and to employ intraoperative chromopertubation to assess for genital tract patency. This systematic approach will facilitate optimal endometriosis management, guide concomitant adnexal surgeries, and enhance post-surgical fertility outcomes. Cancer Research A Permanent Window for Investigating Cancer Metastasis to the Lung Lucia Borriello*1,2, Brian Traub*1,2,3, Anouchka Coste1,2,3, Maja H. Oktay1,2,4,5, David Entenberg1,2,4 1Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center, 2Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center, 3Department of Surgery, Einstein College of Medicine / Montefiore Medical Center, 4Integrated Imaging Program, Einstein College of Medicine / Montefiore Medical Cente, 5Department of Pathology, Einstein College of Medicine / Montefiore Medical Center Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells. Cancer Research Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis George S. Karagiannis1,2,3, Jessica M. Pastoriza1,2,4, Lucia Borriello1,2, Rojin Jafari1,2, Anouchka Coste1,2,4, John S. Condeelis1,2,3,4, Maja H. Oktay1,2,3,5, David Entenberg1,2,3 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Integrated Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.
