Fleischer Institute of Diabetes and Metabolism 1 article published in JoVE Genetics Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications Gabrielle J. Benitez1, Kosaku Shinoda1,2,3 1Departments of Medicine, Albert Einstein College of Medicine, 2Departments of Molecular Pharmacology, Albert Einstein College of Medicine, 3Fleischer Institute of Diabetes and Metabolism This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.
