3 articles published in JoVE
Dissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling Michelle J. Veite-Schmahl1, Daniel P. Regan1, Adam C. Rivers1, Joseph F. Nowatzke1,2, Michael A. Kennedy1 1Department of Chemistry & Biochemistry, Miami University, 2College of Medicine, Central Michigan University This video article provides a detailed demonstration of the procedures required to successfully remove the pancreas from a mouse by dissection for histological analysis and metabolic profiling.
Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum David M. Linz*1, Courtney M. Clark-Hachtel*1, Ferran Borràs-Castells*1, Yoshinori Tomoyasu1 1Department of Biology, Miami University RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential Jenna M. Dolhi1, Nicholas Ketchum1, Rachael M. Morgan-Kiss1 1Department of Microbiology, Miami University Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.