2 articles published in JoVE
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles Catherine B. Anders1, Joshua D. Baker1, Adam C. Stahler1, Austin J. Williams1, Jackie N. Sisco2, John C. Trefry2, Dawn P. Wooley2, Ioana E. Pavel Sizemore1 1Department of Chemistry, Wright State University, 2Department of Neuroscience, Cell Biology, and Physiology, Wright State University Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats Saeed Alshahrani1, Filipe Fernandez-Conti2, Amanda Araujo2, Mauricio DiFulvio1 1Department of Pharmacology and Toxicology, Wright State University, 2Human Movement Laboratory, Universidade São Judas Tadeu Here, we describe a rapid reliable and simple procedure to determine the lowest temperature at which rats or mice show nocifensive behavior, i.e. the thermal nociceptive threshold (TNT). This method applies a slowly increasing thermal stimulus allowing precise and reproducible estimation of TNTs with minimum, if any, stress to the animals.