2 articles published in JoVE
Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells Raffaella Spina1, Dillon M. Voss1, Laura Asnaghi2, Andrew Sloan1,3, Eli E. Bar1 1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine An efficient screening protocol is presented for the identification of small molecules that promote astroglial differentiation in glioblastoma stem cells (GSCs). The assay is based on a stem cell differentiation reporter whereby the expression of the enhanced GFP (eGFP) is driven by the human GFAP promoter.
Ileectomy-induced Bile Overaccumulation in Mouse Intestine Rongli Zhang*1,2,3, Jonathan W. Ray1,2, Mukesh K. Jain1,2, Shuxin Han*1,2 1Department of Medicine, Case Cardiovascular Research Institute, Case Western Reserve University, 2Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, 3Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine, University Hospitals Cleveland Medical Center Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.