University Hospitals Cleveland Medical Center 4 articles published in JoVE Biology Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria Dionysios C. Watson1,2,3, Sadie Johnson1, Akeem Santos1,4, Mei Yin5, Defne Bayik1, Justin D. Lathia1,3, Mohammed Dwidar1,3,4 1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health, Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research. Bioengineering Optical Imaging of Isolated Murine Ventricular Myocytes Shuxin Han1,2, Matt Klos3, Sherry Morgenstern3, Ramiz Ahmad3, Isabella Pua3, Shreyas Suresh1, Kayla Hicks3, Eric Devaney3 1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements. Cancer Research Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells Raffaella Spina1, Dillon M. Voss1, Laura Asnaghi2, Andrew Sloan1,3, Eli E. Bar1 1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine An efficient screening protocol is presented for the identification of small molecules that promote astroglial differentiation in glioblastoma stem cells (GSCs). The assay is based on a stem cell differentiation reporter whereby the expression of the enhanced GFP (eGFP) is driven by the human GFAP promoter. Medicine Ileectomy-induced Bile Overaccumulation in Mouse Intestine Rongli Zhang*1,2,3, Jonathan W. Ray1,2, Mukesh K. Jain1,2, Shuxin Han*1,2 1Department of Medicine, Case Cardiovascular Research Institute, Case Western Reserve University, 2Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, 3Institute for Transformative Molecular Medicine, Case Western Reserve University School of Medicine, University Hospitals Cleveland Medical Center Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.
