Cincinnati Children’s Hospital Medical Center 4 articles published in JoVE Bioengineering Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality Gauri Kulkarni*1, Athanasia Apostolou*1, Lorna Ewart1, Carolina Lucchesi1, Magdalena Kasendra2,3 1Emulate Inc. Boston, 2Center for Stem Cell and Organoid Medicine, Cincinnati Children’s Hospital Medical Center, 3Division of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center Biopsy-derived intestinal organoids and organ-on-a-chips technologies are combined into a microphysiological platform to recapitulate region-specific intestinal functionality. Developmental Biology Studying Wnt Signaling During Patterning of Conducting Airways John Snowball1, Manoj Ambalavanan1, Debora Sinner1,2 1Neonatology and Pulmonary Biology-Perinatal Institute, Cincinnati Children’s Hospital Medical Center, 2University of Cincinnati, College of Medicine The use of reporter mice coupled to whole mount and section staining, microscopy and in vivo assays facilitates the analysis of mechanisms underlying the normal patterning of the respiratory tract. Here we describe how these techniques contributed to the analysis of Wnt signaling during tracheal development. Developmental Biology Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration Chandrima Sinha*1,2, Kavisha Arora*1, Anjaparavanda P. Naren1,2 1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center MRP4 regulates various cyclic nucleotide-dependent signaling events including a recently elucidated role in cell migration. We describe a direct, but multifaceted approach to unravel the downstream molecular targets of MRP4 resulting in identification of a unique MRP4 interactome that plays key roles in the fine-tuned regulation of fibroblast migration. Biology A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors Meenu Kesarwani1, Erika Huber1, Zachary Kincaid1, Mohammad Azam1 1Divisions of Experimental Hematology and Cancer Pathology, Cancer Blood Disease Institute, Cincinnati Children's Hospital Medical Center Emergence of genetic resistance against kinase inhibitor therapy poses significant challenge for effective cancer therapy. Identification and characterization of resistant mutations against a newly developed drug helps in better clinical management and next generation drug design. Here, we describe our protocol for in vitro screening and validation of resistant mutations.
