3 articles published in JoVE
Characterization of the Sense of Agency over the Actions of Neural-machine Interface-operated Prostheses Jonathon S. Schofield1, Courtney E. Shell1, Zachary C. Thumser1,2, Dylan T. Beckler1, Raviraj Nataraj3, Paul D. Marasco1,4 1Laboratory for Bionic Integration, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 2Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 3Department of Biomedical Engineering, Stevens Institute of Technology, 4Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center Here we present a protocol which characterizes the sense of agency developed over the control of sensate virtual or robotic prosthetic hands. Psychophysical questionnaires are employed to capture the explicit experience of agency, and time interval estimates (intentional binding) are employed to implicitly measure the sense of agency.
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos Jianhong Lin*1,2, Yi Hu*1, Jian-Jun Zhao1 1Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 2Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School This manuscript describes the synthesis of a single-wall carbon nanotube (SWCNT)-conjugated MALAT1 antisense gapmer DNA oligonucleotide (SWCNT-anti-MALAT1), which demonstrates the reliable delivery of the SWCNT and the potent therapeutic effect of anti-MALAT1 in vitro and in vivo. Methods used for synthesis, modification, conjugation, and injection of SWCNT-anti-MALAT1 are described.
Ferric Chloride-induced Murine Thrombosis Models Wei Li1,2, Marvin Nieman3, Anirban Sen Gupta4 1Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 2Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, 3Department of Pharmacology, Case Western Reserve University, 4Department of Biomedical Engineering, Case Western Reserve University We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation.