Lerner Research Institute, Cleveland Clinic 7 articles published in JoVE Cancer Research Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture Swetha J. Sundar1, Sajina Shakya2, Violette Recinos1, Christopher G. Hubert2 1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo. Engineering The Immersive Cleveland Clinic Virtual Reality Shopping Platform for the Assessment of Instrumental Activities of Daily Living Jay L. Alberts1,2, Morgan McGrath1,3, Mandy Miller Koop1, Colin Waltz1, Logan Scelina1, Kathryn Scelina1, Anson B. Rosenfeldt1 1Department of Biomedical Engineering, Cleveland Clinic, Lerner Research Institute, 2Neurological Institute, Center for Neurological Restoration, Cleveland Clinic, 3Case Western Reserve University, School of Medicine Virtual reality (VR) is a powerful yet underutilized approach to advance the diagnosis and treatment of neurological disease. The Cleveland Clinic Virtual Reality Shopping platform combines state-of-the-art VR content with an omnidirectional treadmill to quantify instrumental activities of daily living-a proposed prodromal marker of neurological disease. Neuroscience Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions Maksim Sinyuk1, Jessica L. Williams1,2 1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems. Neuroscience Comprehensive Autopsy Program for Individuals with Multiple Sclerosis Ranjan Dutta*1, Kedar R. Mahajan*1,3, Kunio Nakamura*3, Daniel Ontaneda*2, Jacqueline Chen1, Christina Volsko1, Jessica Dudman1, Emilie Christie1, Jordon Dunham1, Robert J. Fox3, Bruce D. Trapp1 1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, 2Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 3Mellen Center for Treatment and Research in Multiple Sclerosis, Neurological Institute, Cleveland Clinic Multiple sclerosis is an inflammatory demyelinating disease with no cure. Analysis of brain tissue provides important clues to understanding the pathogenesis of the disease. Here we discuss the methodology and downstream analysis of MS brain tissue collected through a unique rapid autopsy program in operation at the Cleveland Clinic. Behavior Characterization of the Sense of Agency over the Actions of Neural-machine Interface-operated Prostheses Jonathon S. Schofield1, Courtney E. Shell1, Zachary C. Thumser1,2, Dylan T. Beckler1, Raviraj Nataraj3, Paul D. Marasco1,4 1Laboratory for Bionic Integration, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 2Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 3Department of Biomedical Engineering, Stevens Institute of Technology, 4Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center Here we present a protocol which characterizes the sense of agency developed over the control of sensate virtual or robotic prosthetic hands. Psychophysical questionnaires are employed to capture the explicit experience of agency, and time interval estimates (intentional binding) are employed to implicitly measure the sense of agency. Cancer Research Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos Jianhong Lin*1,2, Yi Hu*1, Jian-Jun Zhao1 1Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 2Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School This manuscript describes the synthesis of a single-wall carbon nanotube (SWCNT)-conjugated MALAT1 antisense gapmer DNA oligonucleotide (SWCNT-anti-MALAT1), which demonstrates the reliable delivery of the SWCNT and the potent therapeutic effect of anti-MALAT1 in vitro and in vivo. Methods used for synthesis, modification, conjugation, and injection of SWCNT-anti-MALAT1 are described. Medicine Ferric Chloride-induced Murine Thrombosis Models Wei Li1,2, Marvin Nieman3, Anirban Sen Gupta4 1Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, 2Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, 3Department of Pharmacology, Case Western Reserve University, 4Department of Biomedical Engineering, Case Western Reserve University We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation.
