3 articles published in JoVE
Single-cell Microfluidic Analysis of Bacillus subtilis Matthew T. Cabeen1, Richard Losick2 1Department of Microbiology and Molecular Genetics, Oklahoma State University, 2Department of Molecular and Cellular Biology, Harvard University We present a method for the microfluidic analysis of individual bacterial cell lineages using Bacillus subtilis as an example. The method overcomes shortcomings of traditional analytical methods in microbiology by allowing observation of hundreds of cell generations under tightly controllable and uniform growth conditions.
Electrophysiological Recording in the Brain of Intact Adult Zebrafish Lindsey Johnston1, Rebecca E. Ball2, Seth Acuff3, John Gaudet4, Andrew Sornborger5, James D. Lauderdale2 1Department of Genetics, University of Georgia, 2Department of Cellular Biology, University of Georgia, 3College of Engineering, Architecture and Technology, Oklahoma State University, 4University of Georgia, 5Department of Mathematics, University of California, Davis This paper describes how an adult zebrafish can be immobilized, intubated, and used for in vivo electrophysiological experiments to allow recordings and manipulation of neural activity in an intact animal.
RNA Interference in Ticks Katherine M. Kocan1, Edmour Blouin1, José de la Fuente1,2 1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 2(CSIC-UCLM-JCCM), Instituto de Investigación en Recursos Cinegéticos IREC A method for RNA interference (RNAi) by injection of dsRNA into unfed ticks is described. RNAi is the most widely used gene-silencing technique in ticks where the use of other methods of genetic manipulation has been limited.