University of Oklahoma College of Medicine 1 article published in JoVE Biology Targeted DNA Methylation Analysis by Next-generation Sequencing Dustin R. Masser1, David R. Stanford1, Willard M. Freeman1,2 1Department of Physiology, University of Oklahoma College of Medicine, 2Department of Geriatric Medicine, University of Oklahoma College of Medicine Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.
